InSight
-在显微镜上升级光场成像功能
-单次/实时 3D显微成像
-数字聚焦扩展视场景深
Insight
超出现有显微镜的成像极限
大多数3D显微成像技术是通过对样品的位置深度进行扫描来实现；由于生物样品动力学研究和对光敏感的苛刻限制，Insight采用非扫描技术，实现单次3D成像采集，使样品尽量避免光毒性或光漂白，从而确保在高时间分辨率的条件下采集3D动态事件。
工作原理
不同于传统扫描系统扫描样品时所需要移动的时间限制，Insight采用非样品扫描方式，该技术基于高分辨率光场成像技术和PhaseView采集和软件引擎。
Phaseview光场成像存储空间和角度信息，而且不会向典型的小透镜阵列损失个别性能。
这种新方法集合了可变焦距的镜头和微透镜整列。如下图所示：
4D的数据将通过Phaseview的软件进行处理和分析。
InSight技术参数：
InSight LFM（add-on for any microscope)
| Camera compatibility (camera not included) | Format >=1" |
| Microscope Interface | Video Port 1x C-mount adapter |
Physical Dimensions(mm),
Weight(g) | Insight Head:
245(H) 56(W) 80(D), 470g
Control Unit:
40(H) 160(W) 150(D), 150g |
InSight LFC(with embedded camera)
| Camera | Scientiic CMOS 13.31mmx13.31mm |
| Sensor Specs | 2048x2048
6.5um x 6.5um |
| Full well capacity | 30 000 electrons |
| Dynamic | 33000:1 |
| Frame rate(full resolution) | Frame rate(full resolution) |
工作模式：
Scanless Mode （Light Field）
Z range and Z resolution are objective dependant
Z Range = 100 x Objective Depth of Field
Z Resolution = Z Range / 10
Scanning Mode
Z range and Z step are objective dependant
Z Range = 16mm / (G_Obj)²
Z Step = Z Range / 2000
典型应用：
High-speed volumetric imaging of weak fluorescent specimens
In-vivo imaging of C. elegans, Drosophila and zebrafish
3D functional imaging of neuronal activity
High-speed Ca2+ imaging
Fluorescence and brightfield microscopy
Live Imaging of cellular dynamics in three dimensions
Trajectory monitoring of microscopic objects
Instant 3D recording of dynamic events or moving specimens
